TIM-3 Antibody / HAVCR2
TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+Tc1) but not on helper T cells (CD4+Th2 and CD8+Tc2) . In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry: 1-2 µg/mL
UniProt
Q8TDQ0
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 22-202) was used as the immunogen for this TIM-3 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
TIM3/2399
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the TIM-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TIM-3 antibody is available for research use only.
Storage Conditions
Store the TIM-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the TIM-3 antibody should be determined by the researcher.
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