Napsin A Antibody

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

O96009

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 189-299 from the human protein were used as the immunogen for the Napsin A antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

NAPSA/3305

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Napsin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Napsin A antibody is available for research use only.

Storage Conditions

Store the Napsin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Napsin A antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human lung adenocarcinoma with Napsin A antibody (clone NAPSA/3305) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.