B7-H6 Antibody

B7-H6 (NCR3LG1) is a transmembrane endogenous ligand expressed on the surfaces of tumor cells. B7-H6 binds with NKp30 present on NK cells. Binding of B7-H6 to NKp30 causes the ligation of NKp30 subsequently inducing NK cell activation and target cell cytolysis. B7-H6 is not constitutively expressed on normal tissue. Whether it can be expressed endogenously under certain conditions is under investigation. B7-H6 has been detected on circulating pro-inflammatory CD14 (+) CD16 (+) monocytes in some patients with sepsis.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q68D85

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 38-169 from the human protein was used as the immunogen for the B7-H6 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

B7H6/4821

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the B7-H6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This B7-H6 antibody is available for research use only.

Storage Conditions

Store the B7-H6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the B7-H6 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human tonsil with B7-H6 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.