Beta Amyloid Antibody

Proteolytic cleavage of the Amyloid protein precursor (APP) gives rise to the /A4 Amyloid protein. This protein is involved in the formation of neurofibrillary tangles and plaques that characterize the senile plaques of Alzheimer s patients. APLP1 (Amyloid precursor-like protein 1) and APLP2 are structurally similar to APP. Human APLP2 is a membrane-bound sperm protein that contains a region highly homologous to the transmembrane-cytoplasmic domains of APP found in brain plaques of Alzheimer s disease patients.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P05067

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 578-680 from the human protein was used as the immunogen for the Beta Amyloid antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

APP/3343

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Beta Amyloid antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Beta Amyloid antibody is available for research use only.

Storage Conditions

Store the Beta Amyloid antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Beta Amyloid antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human brain with Beta Amyloid antibody (clone APP/3343) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.