MSLN Antibody / Mesothelin

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT, Western blot: 2-4 µg/mL

UniProt

Q13421

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 273-407 from the human protein was used as the immunogen for the MSLN antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

MSLN/3387

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the MSLN antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MSLN antibody is available for research use only.

Storage Conditions

Store the MSLN antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the MSLN antibody should be determined by the researcher.

Location

Cytoplasmic, cell surface, secreted

Image Legend

IHC staining of FFPE human mesothelioma with MSLN antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.