Recombinant SOX11 Antibody

Recognizes a protein of ~47kDa, identified as SOX11. This MAb is highly specific and does not cross-react with other members of the SOX-family. Mantle cell lymphoma (MCL) accounts for 5% to 10% of mature B-cell neoplasms and is an aggressive disease genetically characterized by overexpression of Cyclin D1. Cyclin D1 overexpression is the hallmark of MCL. However, approximately 5%-10% of MCLs lack Cyclin D1 expression and may be misdiagnosed. Almost all Cyclin D1-positive as well as Cyclin D1-negative MCL, show overexpression of SOX-11. The detection of this transcription factor is a useful biomarker for identifying true cyclin D1-negative MCL.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

UniProt

P35716

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 300-400 from the human protein was used as the immunogen for the recombinant SOX11 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

SOX11/3235R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant SOX11 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant SOX11 antibody is available for research use only.

Storage Conditions

Store the recombinant SOX11 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant SOX11 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

IHC staining of FFPE human mantle cell lymphoma with recombinant SOX11 antibody (clone SOX11/3235R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.