PRKCA Antibody / PKC alpha
Protein Kinase C alpha (PKC) is involved in the regulation of cell proliferation during cell cycle progression. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Clone 133 recognizes the isoform of PKC and binds to a sequence at the C terminus of PKC to detect its expression in vitro.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P17252
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Full-length protein was used as the immunogen for the PRKCA antibody. The epitope has been mapped to amino acids PQFVHPILQSAV at the C terminus.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
133
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the PRKCA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PRKCA antibody is available for research use only.
Storage Conditions
Store the PRKCA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the PRKCA antibody should be determined by the researcher.
Location
Cytoplasm, cell membrane, nucleus
Image Legend
Frequently Asked Questions
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