PRKCA Antibody / PKC alpha

Protein Kinase C alpha (PKC) is involved in the regulation of cell proliferation during cell cycle progression. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Clone 133 recognizes the isoform of PKC and binds to a sequence at the C terminus of PKC to detect its expression in vitro.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P17252

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Full-length protein was used as the immunogen for the PRKCA antibody. The epitope has been mapped to amino acids PQFVHPILQSAV at the C terminus.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

133

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the PRKCA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PRKCA antibody is available for research use only.

Storage Conditions

Store the PRKCA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the PRKCA antibody should be determined by the researcher.

Location

Cytoplasm, cell membrane, nucleus

Image Legend

IHC staining of FFPE human tonsil with PRKCA antibody (clone 133) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.