Myelin Basic Protein Antibody
Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4274 recognizes an epitope in the 36-50 region of MBP, useful in clinical diagnosis to detect MBP levels or myelination in human MBP.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P02686
Host
Rat
Reactivity
Human
Immunogen
Amino acids GILDSIGRFFGGDRG from the human protein were used as the immunogen for the Myelin Basic Protein antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
MBP/4274
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Myelin Basic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Myelin Basic Protein antibody is available for research use only.
Storage Conditions
Store the Myelin Basic Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Myelin Basic Protein antibody should be determined by the researcher.
Location
Cell surface, cytoplasm
Image Legend
Frequently Asked Questions
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