Myelin Basic Protein Antibody

Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4274 recognizes an epitope in the 36-50 region of MBP, useful in clinical diagnosis to detect MBP levels or myelination in human MBP.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P02686

Host

Rat

Reactivity

Human

Immunogen

Amino acids GILDSIGRFFGGDRG from the human protein were used as the immunogen for the Myelin Basic Protein antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

MBP/4274

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Myelin Basic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Myelin Basic Protein antibody is available for research use only.

Storage Conditions

Store the Myelin Basic Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Myelin Basic Protein antibody should be determined by the researcher.

Location

Cell surface, cytoplasm

Image Legend

IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4274) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.