MBP Antibody

Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin. MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4272 recognizes an epitope in the 119-131 region of MBP, useful in clinical diagnosis to detect MBP levels in human, rat and cow MBP.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P02686

Host

Mouse

Reactivity

Human

Immunogen

Amino acids GAEGQRPGFGYGG were used as the immunogen for the MBP antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

MBP/4272

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the MBP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MBP antibody is available for research use only.

Storage Conditions

Store the MBP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the MBP antibody should be determined by the researcher.

Location

Cell surface, cytoplasm

Image Legend

IHC staining of FFPE human brain with MBP antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.