Aromatase Antibody / CYP19A1

CYP450 Aromatase is part of the CYP19A1 family involved in the aromatization of androgens to estrogens, a highly conserved mechanism amongst mammals. Clone CYP19A1/4257 recognizes a conserved epitope on the CYP450 aromatase, allowing for detection of aromatase levels using various analysis methods.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: order Ab without BSA for coating, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

O14793

Host

Mouse

Reactivity

Human

Immunogen

Amino acids KALEDDVIGYPVKK were used as the immunogen for the Aromatase antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

CYP19A1/4257

Applications

ELISA, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Aromatase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Aromatase antibody is available for research use only.

Storage Conditions

Store the Aromatase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Aromatase antibody should be determined by the researcher.

Location

Membrane

Image Legend

IHC staining of FFPE human placenta with Aromatase antibody (clone CYP19A1/4257) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.