Recombinant MART-1 Antibody / Melan-A

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q16655

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human MART-1 protein was used as the immunogen for the recombinant MART-1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

MLANA/4475R

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant MART-1 antibody is available for research use only.

Storage Conditions

Store the recombinant MART-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant MART-1 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of human COLO-38 cell lysate with recombinant MART-1 antibody (clone MLANA/4475R) . Expected molecular weight ~20 kDa with possible doublet.