Recombinant Type II Cytokeratin Antibody (CK1-8)
The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
Q01546
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 300-400 from Type II Cytokeratins was used as the immunogen for the recombinant Type II Cytokeratin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
KRTH/4392R
Type
Recombinant
Applications
IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant Type II Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Type II Cytokeratin antibody is available for research use only.
Storage Conditions
Store the recombinant Type II Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Type II Cytokeratin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Frequently Asked Questions
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