Recombinant Cyclin D1 Antibody
Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P24385
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 200-295 from the human protein was used as the immunogen for the recombinant Cyclin D1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2a κ
Clone
RCCND1/4752
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant Cyclin D1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Cyclin D1 antibody is available for research use only.
Storage Conditions
Store the recombinant Cyclin D1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Cyclin D1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of mouse RAW264.7 cell lysate with recombinant Cyclin D1 antibody. Predicted molecular weight: 32-36 kDa.
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