Recombinant Synaptophysin Antibody

This monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

IDP08247

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 224-313 from the human protein was used as the immunogen for the recombinant Synaptophysin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2a κ

Clone

RSYP/4654

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant Synaptophysin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Synaptophysin antibody is available for research use only.

Storage Conditions

Store the recombinant Synaptophysin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Synaptophysin antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human pancreas with recombinant Synaptophysin antibody (clone rSYP/4654) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.