Recombinant MUC1 Antibody / Mucin-1
Mucin-1/MUC1/EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Anti-EMA antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and colorectal carcinoma. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms such as schwannomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P15941
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human MUC1 protein was used as the immunogen for the recombinant MUC1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2a κ
Clone
RMUC1/4418
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant MUC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant MUC1 antibody is available for research use only.
Storage Conditions
Store the recombinant MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant MUC1 antibody should be determined by the researcher.
Location
Cytoplasmic, cell surface
Image Legend
Western blot testing of human MCF7 cell lysate with MUC1 antibody (clone rMUC1/4418) . This glycoprotein is commonly visualized between 120~500 kDa.
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