HER2 Antibody / ErbB2 / Extracellular region
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
IDP04626
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant protein encoding the extracellular domain of human c-erbB2 was used as the immunogen for the HER2 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
ERBB2/4439R
Applications
FACS, IF, IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HER2 antibody is available for research use only.
Storage Conditions
Store the HER2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the HER2 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
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