HER2 Antibody / ErbB2 / Extracellular region

Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

IDP04626

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant protein encoding the extracellular domain of human c-erbB2 was used as the immunogen for the HER2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ERBB2/4439R

Applications

FACS, IF, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HER2 antibody is available for research use only.

Storage Conditions

Store the HER2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the HER2 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ERBB2/4439R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.