P16INK4a Antibody / CDKN2A

P16INK4a is a tumor suppressor protein. It is a specificinhibitor of cdk4/cdk6, and a tumorsuppressor involved inthe pathogenesis of a variety of malignancies. Recentanalyses of the p16INK4a gene revealed homozygousdeletions, nonsense, missense, or frameshift mutations inseveral human cancers. Although the frequency of p16INK4aabnormalities is higher in tumor derived cell lines than inunselected primary tumors, significant subsets of clinicalcases with aberrant p16INK4a gene have been reportedamong melanomas, gliomas, esophageal, pancreatic, lung, and urinary bladdercarcinomas, and some types of leukemia.Expression of p16INK4a (p16 positive) is highly correlatedwith human papilloma virus (HPV) infection in head and necksquamous cell carcinomas (HNSCC) . p16 status is animportant prognostic indicator in HNSCC and the p16positive/HPV16 negative group is likely a distinct subgrouplacking any HPV genotype.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P42771

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the p16INK4a antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

CDKN2A/4499

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the p16INK4a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This p16INK4a antibody is available for research use only.

Storage Conditions

Store the p16INK4a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the p16INK4a antibody should be determined by the researcher.

Location

Nuclear and cytoplasmic

Image Legend

IHC staining of FFPE human cervix with p16INK4a antibody (clone CDKN2A/4499) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.