Recombinant Prostein Antibody / SLC45A3

Human prostein is a 553 aa protein identified by cDNA library subtraction and subsequent high throughput microarray screening of prostate cancer. Prostein has multiple transmembrane domains. Prostein has been shown to be uniquely expressed in normal and cancerous prostatic tissues. By immunohistochemistry, prostein is expressed in the vast majority of normal and malignant prostatic tissues, regardless of grade and metastatic status. No protein expression is detected in normal and malignant tissue samples representing the great majority of essential tissues and tumors. In particular, prostein is expressed in most of poorly differentiated prostatic carcinoma, including small cell prostate carcinoma. Prostein is more specific and sensitive for prostatic carcinomas than PSA and PSAP.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q96JT2

Host

Rabbit

Reactivity

Human

Immunogen

An N terminus region peptide from the human protein was used as the immunogen for the recombinant Prostein antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

ZR9

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant Prostein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Prostein antibody is available for research use only.

Storage Conditions

Store the recombinant Prostein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Prostein antibody should be determined by the researcher.

Location

Membrane, Vesicles, Nucleus

Image Legend

IHC staining of FFPE human prostate carcinoma with recombinant Prostein antibody (clone ZR9) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.