Recombinant ZAP70 Antibody
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Product Specifications
CAS Number
9000-83-3
Specifications
ELISA: 2-4 µg/mL for coating (order Ab without BSA), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P43403
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 247-382 from the human protein was used as the immunogen for the recombinant ZAP70 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
ZAP70/3921R
Type
Recombinant
Applications
ELISA, IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant ZAP70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant ZAP70 antibody is available for research use only.
Storage Conditions
Store the recombinant ZAP70 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant ZAP70 antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Beta Arrestin 2 (ARRB2) (NM_004313) Human Tagged ORF Clone Lentiviral Particle
