OX40 Antibody

This MAb recognizes a protein of 43kDa, identified as OX40, which is also known as CD134. OX40 is a type I integral membrane glycoprotein and member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family. It is expressed on activated T lymphocytes, hematopoietic precursor cells and fibroblasts. It functions as a T cell co-stimulatory receptor when bound by OX40 Ligand / TNFSF4 that is expressed by antigen presenting cells. OX40 thereby plays roles in T-cell activation as well as the regulation of differentiation, proliferation or apoptosis of normal and malignant lymphoid cells. OX40 is upregulated at the sites of inflammation, especially in case of multiple sclerosis and psoriatic lesions.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: order Ab without BSA for coating, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P43489

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 59-205 from the human protein was used as the immunogen for the OX40 antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

OX40/3427

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the OX40 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This OX40 antibody is available for research use only.

Storage Conditions

Store the OX40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the OX40 antibody should be determined by the researcher.

Location

Cytoplasmic, membranous

Image Legend

IHC staining of FFPE human tonsil with OX40 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.