Kindlin-1 Antibody / KIND1

Kindlin-1 (also known as KIND1, KINDLERIN, UNC112-RELATED PROTEIN 1 (URP1) is an important component of cell-extracellular matrix adhesion. Kindlin-1 is involved in cell adhesion, possibly via its interaction with integrins and may mediate TGF-beta 1 signaling during tumor progression. Kindlin-1 has been shown to be significantly upregulated in lung and colon carcinomas. Mutations in the kindlin-1 gene, FERMT1, causes Kindler syndrome. Kindler syndrome is an autosomal recessive disorder characterized by neonatal blistering, sun sensitivity, atrophy, abnormal pigmentation and fragility of the skin. We have developed monoclonal anti-kidlin-1 antibodies. This monoclonal antibody recognizes kindlin-1 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q9BQL6

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 216-677 from the human protein were used as the immunogen for the Kindlin-1 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

4A5.14

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Kindlin-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Kindlin-1 antibody is available for research use only.

Storage Conditions

Store the Kindlin-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Kindlin-1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human liver with Kindlin-1 antibody (clone 4A5.14) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.