A2M Antibody / Alpha-2-Macroglobulin

Alpha-2-Macroglobulin (A2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, it was characterized as a pan-proteinase inhibitor that was able to bait proteinases into cleaving specific peptide sequences on A2M. This interaction induces a conformational change, thus enabling it to trap the proteinase and further inhibit its activity. Subsequently, A2M has been shown to function as a carrier protein and regulator of cytokines during inflammation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P01023

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 604-748 from the human protein was used as the immunogen for the A2M antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

A2M/3623

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the A2M antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This A2M antibody is available for research use only.

Storage Conditions

Store the A2M antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the A2M antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human liver with A2M antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.