A2M Antibody / Alpha-2-Macroglobulin

Alpha-2-Macroglobulin (A2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, it was characterized as a pan-proteinase inhibitor that was able to bait proteinases into cleaving specific peptide sequences on A2M. This interaction induces a conformational change, thus enabling it to trap the proteinase and further inhibit its activity. Subsequently, A2M has been shown to function as a carrier protein and regulator of cytokines during inflammation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P01023

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 604-748 from the human protein was used as the immunogen for the A2M antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

A2M/3623

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the A2M antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This A2M antibody is available for research use only.

Storage Conditions

Store the A2M antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the A2M antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human liver with A2M antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.