TdT Antibody / DNA nucleotidylexotransferase

Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase which catalyzes the addition of deoxyribonucleotides onto the 3 termini of DNA. The human TdT gene maps to chromosome 10q24.1 and encodes a 510 amino acid protein. Human TdT is synthesized as a single chain peptide that elicits a minor preference for incorporation of deoxyribonucleotides over ribonucleotides forming DNA strands. TdT is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL for coating; order Ab without BSA), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P04053

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 52-192 from the human protein was used as the immunogen for the TdT antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

DNTT/1453

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the TdT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TdT antibody is available for research use only.

Storage Conditions

Store the TdT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the TdT antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human thymus with TdT antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.