Cystatin A Antibody / CSTA

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P01040

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-98 from the human protein was used as the immunogen for the Cystatin A antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CSTA/3553

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Cystatin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cystatin A antibody is available for research use only.

Storage Conditions

Store the Cystatin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Cystatin A antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic, nuclear

Image Legend

IHC staining of FFPE human prostate with Cystatin A antibody (clone CSTA/3553) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.