Annexin A1 Antibody

The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P04083

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human Annexin A1 protein was used as the immunogen for the Annexin A1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

ANXA1/3727

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Annexin A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Annexin A1 antibody is available for research use only.

Storage Conditions

Store the Annexin A1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Annexin A1 antibody should be determined by the researcher.

Location

Nucleus, cytoplasm

Image Legend

IHC staining of FFPE human placenta with Annexin A1 antibody (clone ANXA1/3727) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.