Recombinant CD8A Antibody
This MAb is specific for the 32kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) . A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
Product Specifications
CAS Number
9000-83-3
Specifications
ELISA: order Ab without BSA for coating, Flow cytometry: 1-2ug/million cells
UniProt
P01732
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human full-length protein was used as the immunogen for the recombinant CD8A antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
CD8/3892R
Type
Recombinant
Applications
ELISA, FACS
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant CD8A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD8A antibody is available for research use only.
Storage Conditions
Store the recombinant CD8A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CD8A antibody should be determined by the researcher.
Location
Cell surface
Image Legend
Flow cytometry staining of human lymphocyte-gated PBM cells with recombinant CD8A antibody; Red=recombinant CD8A, Gray= isotype control.
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