Recombinant CD4 Antibody
Recognizes a protein of 55kDa, identified as CD4. CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P01730
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human CD4 protein was used as the immunogen for the recombinant CD4 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RC4/206
Type
Recombinant
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant CD4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD4 antibody is available for research use only.
Storage Conditions
Store the recombinant CD4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CD4 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
Immunofluorescent staining of human PBMC with recombinant CD4 antibody (clone rC4/206, green) .
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