CD25 Antibody / IL2RA

Recognizes a protein of 55kDa, identified as CD25 (Workshop IV; Code A27) . CD25 is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and can effectively block IL-2 binding to CD25.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: order Ab without BSA for coating

UniProt

P01589

Host

Mouse

Reactivity

Human

Immunogen

Stimulated human leukocytes were used as the immunogen for the CD25 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

IL2RA/2393

Applications

ELISA

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CD25 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD25 antibody is available for research use only.

Storage Conditions

Store the CD25 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD25 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using CD25 antibody (clone IL2RA/2393) . These results demonstrate the foremost specificity of the IL2RA/2393 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.