Cystatin B Antibody

Cystatin A (also designated STF1, STFA, stefin A or cystatin AS) and cystatin B (also designated PME, CST6, STFB, CPI-B, stefin B and liver thiol proteinase inhibitor) are thiol protease inhibitors that form complexes with papain and the cathepsins B, H, and L. cystatin A, a cytoplasmic protein, is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. cystatin B protects against intracellular proteases leaking out of lysosomes and is primarily expressed in heart, liver and kidney.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P04080

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human Cystatin B protein was used as the immunogen for the Cystatin B antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CPTC-CSTB-3

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Cystatin B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cystatin B antibody is available for research use only.

Storage Conditions

Store the Cystatin B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Cystatin B antibody should be determined by the researcher.

Location

Cytoplasm and nucleus

Image Legend

IHC staining of FFPE human lung with Cystatin B antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.