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Recombinant CD14 Antibody

CD14 is receptor for endotoxin and apoptotic cells. It is expressed by monocytes/histiocytes, Langerhans cell histiocytosis and giant cell tumor. In cultures, a subpopulation of Kaposi sarcoma cells express CD14. In differential diagnosis, CD14 is more specific than CD68 in the identification of monocytes/histiocytes and associated neoplasias.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P08571

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-100 from the human protein was used as the immunogen for the recombinant CD14 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

LPSR/4180R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant CD14 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD14 antibody is available for research use only.

Storage Conditions

Store the recombinant CD14 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant CD14 antibody should be determined by the researcher.

Location

Cell surface, Secreted, Cytoplasmic (Golgi)

Image Legend

IHC staining of FFPE human tonsil with recombinant CD14 antibody (clone LPSR/4180R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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