Recombinant Catenin beta Antibody

Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 0.5-1ug/million cells, ELISA: order Ab without BSA for coating, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P35222

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human beta-Catenin (p120) protein fragment from the human protein was used as the immunogen for the recombinant Catenin beta antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RCTNNB1/1507

Type

Recombinant

Applications

FACS, ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant Catenin beta antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Catenin beta antibody is available for research use only.

Storage Conditions

Store the recombinant Catenin beta antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Catenin beta antibody should be determined by the researcher.

Location

Cell surface and cytoplasmic

Image Legend

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Catenin beta antibody (clone rCTNNB1/1507) ; Red=isotype control, Blue= recombinant Catenin beta antibody.