Vinculin Antibody
This antibody recognizes both Vinculin (125kDa) and meta-Vinculin (135kDa) . Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P18206
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human Vinculin protein was used as the immunogen for the Vinculin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
VCL/3617
Applications
IF, FACS, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Vinculin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Vinculin antibody is available for research use only.
Storage Conditions
Store the Vinculin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Vinculin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of PFA-fixed human U-87 MG cells with Vinculin antibody (clone VCL/3617, green) and Reddot nuclear stain (red) .
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