Recombinant HHV8 Antibody

HHV8 encodes a latent nuclear antigen (LNA), which is the product of the viral gene orf 73. LNA is capable of forming a complex with retinoblastoma susceptibility gene product, which may be related to its oncogenic activity. HHV8 is associated with three different diseases observed in AIDS patients; kaposis sarcoma.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA: for coating use Ab at 1-5 µg/mL (order Ab without BSA), Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Not Applicable

Host

Rabbit

Immunogen

A recombinant protein corresponding to the latent nuclear antigen 1 molecule of HHV8 was used as the immunogen for the recombinant HHV8 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

HHV8/3633R

Type

Recombinant

Applications

ELISA, IF, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant HHV8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant HHV8 antibody is available for research use only.

Storage Conditions

Store the recombinant HHV8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant HHV8 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human Karposi's sarcoma with recombinant HHV8 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.