EGFR Antibody / EGF Receptor

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P00533

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 344-492 from the human protein was used as the immunogen for the EGFR antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

GFR/2596

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This EGFR antibody is available for research use only.

Storage Conditions

Store the EGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the EGFR antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human placenta with EGFR antibody (clone GFR/2596) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.