CA9 Antibody / Carbonic Anhydrase IX

CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, CAIX is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q16790

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 314-410 from the human protein was used as the immunogen for the CA9 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CA9/3406

Applications

FACS, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CA9 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CA9 antibody is available for research use only.

Storage Conditions

Store the CA9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CA9 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

Western blot testing of human HEK293 cell lysate with CA9 antibody. Predicted molecular weight: 50-55 kDa but may be observed at higher molecular weights due to glycosylation.