Carbonic Anhydrase IX Antibody / CA9
Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200) . In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowmans capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
Q16790
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 314-410 from the human protein was used as the immunogen for the Carbonic Anhydrase IX antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
CA9/3405
Applications
FACS, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the Carbonic Anhydrase IX antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Carbonic Anhydrase IX antibody is available for research use only.
Storage Conditions
Store the Carbonic Anhydrase IX antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the Carbonic Anhydrase IX antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Documents
Frequently Asked Questions
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