Carbonic Anhydrase IX Antibody / CA9

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200) . In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowmans capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q16790

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 314-410 from the human protein was used as the immunogen for the Carbonic Anhydrase IX antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CA9/3405

Applications

FACS, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Carbonic Anhydrase IX antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Carbonic Anhydrase IX antibody is available for research use only.

Storage Conditions

Store the Carbonic Anhydrase IX antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Carbonic Anhydrase IX antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

Flow cytometry testing of FPA fixed human U-87 MG cells with Carbonic Anhydrase IX antibody (clone CA9/3405) ; Red=isotype control, Blue= Carbonic Anhydrase IX antibody.