APOB Antibody / Apolipoprotein B
Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated by APOBEC1 (also designated human (or rat) small intestinal apolipoprotein B mRNA editing protein, HEPR or REPR) in hepatic cells to achieve a steady state proportion of edited and unedited RNA molecules. Two forms of apoB are known to circulate in the plasma of mammals. ApoB-100 is a protein primarily synthesized in the liver as a structural component of very low density lipoprotein particles. A truncated form of apoB-100, apoB-48, is synthesized in the small intestine and contains the amino-terminal 2,152 amino acids of the larger protein. This organ-specific partitioning of apoB production is the resultof RNA editing of a common apoB gene.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P04114
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 592-689 from the human protein was used as the immunogen for the APOB antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
APOB/3300
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the APOB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This APOB antibody is available for research use only.
Storage Conditions
Store the APOB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the APOB antibody should be determined by the researcher.
Location
Cytoplasm, secreted
Image Legend
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