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C-Myc Antibody

It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: order Ab without BSA for coating

UniProt

P01106

Host

Mouse

Reactivity

Human

Immunogen

Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA from C-terminus of the human protein was used as the immunogen for the c-Myc antibody. The epitope has been mapped to amino acids EQKLISEEDL.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

9E11.

Applications

ELISA

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the c-Myc antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This c-Myc antibody is available for research use only.

Storage Conditions

Store the c-Myc antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the c-Myc antibody should be determined by the researcher.

Location

Nuclear

Image Legend

SDS-PAGE analysis of purified, BSA-free c-Myc antibody as confirmation of integrity and purity.

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