FGF21 Antibody / Fibroblast Growth Factor 21

Fibroblast growth factor-1 (FGF-1), also designated acidic FGF, and fibroblast growth factor-2 (FGF-2), also designated basic FGF, are members of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (hst/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10-FGF-23. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q9NSA1

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 91-209 from the human protein was used as the immunogen for the FGF21 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

FGF21/3691

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the FGF21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This FGF21 antibody is available for research use only.

Storage Conditions

Store the FGF21 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the FGF21 antibody should be determined by the researcher.

Location

Secreted (extracellular)

Image Legend

IHC staining of FFPE human kidney with FGF21 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.