Recombinant AKT1 Antibody
Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase (s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL, ELISA: order Ab without BSA for coating
UniProt
P31749
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 85-189 from the human protein was used as the immunogen for the recombinant AKT1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
AKT1/3898R
Type
Recombinant
Applications
WB, ELISA
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant AKT1 antibody is available for research use only.
Storage Conditions
Store the recombinant AKT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant AKT1 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic, cell surface
Image Legend
Western blot testing of human MCF7 cell lysate with recombinant AKT1 antibody. Predicted molecular weight ~56 kDa.
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