Estrogen Receptor alpha Antibody

This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P03372

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 129-312 from the human protein was used as the immunogen for the Estrogen Receptor alpha antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ESR1/3564

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Estrogen Receptor alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Estrogen Receptor alpha antibody is available for research use only.

Storage Conditions

Store the Estrogen Receptor alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Estrogen Receptor alpha antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human breast carcinoma with Estrogen Receptor alpha antibody (clone ESR1/3564) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.