Recombinant N-Cadherin Antibody / CDH2 / CD325

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325 and CDH2. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P19022

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the recombinant N-Cadherin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RCDH2/1426

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant N-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant N-Cadherin antibody is available for research use only.

Storage Conditions

Store the recombinant N-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant N-Cadherin antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC staining of FFPE human heart with recombinant N-Cadherin antibody (clone rCDH2/1426) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.