Recombinant Cytokeratin 8/18 Antibody

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18) . This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained. Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P05787; P05783

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human KRT8/18 protein was used as the immunogen for the recombinant Cytokeratin 8/18 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

KRT8.18/2297R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant Cytokeratin 8/18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Cytokeratin 8/18 antibody is available for research use only.

Storage Conditions

Store the recombinant Cytokeratin 8/18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Cytokeratin 8/18 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human colon with recombinant Cytokeratin 8/18 antibody (clone KRT8.18/2297R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.