TdT Antibody / DNA nucleotidylexotransferase

Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase which catalyzes the addition of deoxyribonucleotides onto the 3 termini of DNA. The human TdT gene maps to chromosome 10q24.1 and encodes a 510 amino acid protein. Human TdT is synthesized as a single chain peptide that elicits a minor preference for incorporation of deoxyribonucleotides over ribonucleotides forming DNA strands. It is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL for coating; order Ab without BSA, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P04053

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 52-192 from the human protein was used as the immunogen for the TdT antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

TDT/1393

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the TdT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TdT antibody is available for research use only.

Storage Conditions

Store the TdT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the TdT antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human thymus with TdT antibody (clone TDT/1393) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.