FN1 Antibody / Fibronectin

Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order Ab without BSA for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P02751

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 467-595 from the human protein was used as the immunogen for the FN1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

FN1/2948

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the FN1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This FN1 antibody is available for research use only.

Storage Conditions

Store the FN1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the FN1 antibody should be determined by the researcher.

Location

Connective tissue matrix

Image Legend

IHC staining of FFPE human lung with FN1 antibody (clone FN1/2948) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.